Video Tutorial ============== This section will include access to a test datasest to learn how to use STEGO.R. This will include the video tutorials. Datasets -------- Tutorial directory (https://zenodo.org/records/12665808) that contains the melanoma dataset with the lymph node and tumor single cell sample. The T cell atlas V2 (https://zenodo.org/records/12569981) will be used to demonstrate some of the functionality in Step 4. Analysis and post-analysis section. Quality control (Steps 1 to 3) ------------------------------ If you already have an .rds seurat object, and the TCR data was processed with scRepertoire proceed to Step 3e. to reformat the meta-data and then step 3c (annotation) and 3d (option). STEP 1. ^^^^^^^^ .. youtube:: aRB31LTj_VM :width: 640 :height: 360 STEP 2. ^^^^^^^^ STEP 3a. ^^^^^^^^ STEP 3b. ^^^^^^^^ STEP 3c. ^^^^^^^^ - Why use semi-supervised annotations? - What are the available annotation models in STEGO.R? - How to do a custom annotation? STEP 3d. ^^^^^^^^ STEP 3e. ^^^^^^^^ Analysis -----------