Video Tutorial

This section will include access to a test datasest to learn how to use STEGO.R.

This will include the video tutorials.

Datasets

Tutorial directory (https://zenodo.org/records/12665808) that contains the melanoma dataset with the lymph node and tumor single cell sample.

The T cell atlas V2 (https://zenodo.org/records/12569981) will be used to demonstrate some of the functionality in Step 4. Analysis and post-analysis section.

Quality control (Steps 1 to 3)

If you already have an .rds seurat object, and the TCR data was processed with scRepertoire proceed to Step 3e. to reformat the meta-data and then step 3c (annotation) and 3d (option).

STEP 1.

STEP 2.

STEP 3a.

STEP 3b.

STEP 3c.

  • Why use semi-supervised annotations?

  • What are the available annotation models in STEGO.R?

  • How to do a custom annotation?

STEP 3d.

STEP 3e.

Analysis